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Western Blotting technical library

Quantitative & Qualitative Western blotting

- Quantitative Fluorescent western blotting

- Chemiluminescent & chemifluorescent Western blotting

- Chemiluminescent & chemifluorescent Western blotting - Glycoprotein, phosphorylation and protein biotinylation detection systems

- Introduction to the total staining protein product Deep Purple™ video



Instrumentation and tools

- 1D equipment: electrophoresis, transfer

- Imaging


Quantitative & Qualitative Western blotting
Powerful solutions for Western blotting

- Quantitative Fluorescent western blotting
ECL Plex western blotting system

ECL Plex detection system: new fluorescent Cy2 and Cy3 conjugates for multiplexed protein detection

Multiplex protein detection using the ECL Plex fluorescent western blotting system

Multiplex protein detection with the ECL Plex fluorescent western blotting system using the Ettan DIGE Imager

Multiplexed protein detection in 2-D gel electrophoresis using the Amersham ECL Plex fluorescent western blotting system

New Amersham ECL Plex Cy2 conjugates for multiplex western blotting on the Storm Imager

New and improved ECL Plex Cy3 conjugates for multiplex western blotting

Visualization of proteins electro-blotted on Hybond ECL and Hybond-P using Deep Purple total protein stain



- Chemiluminescent & chemifluorescent Western blotting
A guide to membrane blocking conditions with ECL Western blotting - TechTip 136

Comparasion of ECL Advance western blotting kit with super signal west femto maximum sensitivity substrate

Detection of 2D Western Blots using ECL and ECL Plus

ECL DualVue Western Blotting Markers ECL

ECL, ECL Plus Western blotting detection systems

Enhanced Chemiluminescence Compared With 125I Detection of Proteins - TechTip 118

Estimating protein molecular weight using ECL DualVue Western Blotting Markers

Immunoscreening Using ECL - TechTip 133

Immunoprecipitation with ECL western blotting using unlabelled cell lysates - TechTip 156

Optimizing ECL Western blotting - TechTip 129

Quantitation of Western Blots using Enhanced Chemiluminescence (ECL)

Sequential Labelling Western blots with Enhanced Chemiluminescence (ECL) - TechTip 120

Strategy for stripping Western Blots and reprobing with ECL Western blotting

Stripping Western Blots for reprobing with Enhanced Chemiluminescence


- Chemiluminescent & chemifluorescent Western blotting - Glycoprotein, phosphorylation and protein biotinylation detection systems
ECL and ECL Plus detection of RPN2220 Phosphorylation Kit

Use of ECL glycoprotein detection system to detect cell surface antigens using biotinylation and immunoprecipitation

Use of ECL glycoprotein detection system to detect glycoproteins after treatment with endoglycosidase-H

Use of ECL glycoprotein detection system to detect glycoproteins after treatment with peptide N-glycosidase F

Use of the ECL glycoprotein detection system and ECL protein biotinylation system to detect total protein and sialic acid groups on glycoproteins after treatment with neuraminidase

Use of the ECL protein biotinylation system for biotinylation of primary antibodies, for the detection of immunoprecipitation - TechTip 146


- Introduction to the total staining protein product Deep Purple™ video
Click here to launch the video.




Instrumentation and tools - 1D equipment: electrophoresis, transfer
ECL Semi-dry Blotters

Get a jump ahead on your western blots and gels with the new Processor Plus

madetoworktogether: for electrophoresis results you can count on



Instrumentation and tools - Imaging
Ettan DIGE Imager

ImageQuant RT ECL Imager

Time for a new image? A complete range of imaging solutions