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Affinity Chromatography, Principles & Methods

This handbook describes the role of affinity chromatography in the purification of biomolecules, the principle of the technique, the media available and how to select them, application examples and ...

Antibody Purification Handbook

The purpose of this handbook is to present the most effective and most frequently used strategies for sample preparation and purification of the many different forms of antibodies and antibody ...

Gel Filtration, Principles and Methods

Gel filtration is a robust technique that is well suited to handling biomolecules that are sensitive to changes in pH, concentration of metal ions or co-factors and harsh environmental conditions. ...

GST Gene Fusion System

This handbook describes the Glutathione S-transferase (GST) Gene Fusion System which is a versatile system for the expression, purification and detection of fusion proteins produced in Escherichia ...

Hydrophobic Interaction and Reversed Phase Chromatography, Principles and Methods

This handbook describes both theoretical and practical aspects of the technique, the media available and how to select them, together with application examples and detailed instructions for the most ...

Ion Exchange Chromatography & Chromatofocusing, Principles and Methods

IEX for the separation of biomolecules was introduced in the 1960s and continues to play a major role in the separation and purification of biomolecules. Today, IEX is one of the most requently ...

Protein Purification Handbook

The development of techniques and methods for protein purification has been an essential pre-requisite for many of the advancements made in biotechnology. The key to successful and efficient protein ...

Purifying Challenging Proteins - Principles and Methods

This handbook is intended for students and experienced researchers with an interest in the isolation of integral membrane proteins, multiprotein complexes, or in refolding proteins from inclusion ...

Recombinant Protein Purification Handbook - Principles and Methods

This handbook is intended for those interested in the expression and purification of recombinant proteins. The use of recombinant proteins has increased greatly in recent years, as has the wealth of ...

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