The diversity of the antibody-antigen interaction and the ability to manipulate the characteristics of the interaction has created many uses for antibodies and antibody fragments, both for immunochemical techniques within general research and for therapeutic and diagnostic applications. The Antibody Purification Handbook presents the tools, strategies, and solutions to effectively purify the major classes of antibodies and antibody fragments using GE Healthcare products. Advice is given on how to:
Prepare samples for purification
Plan simple, one-step and more advanced, multistep purifications
Remove contaminants
Scale-up an antibody purification
Select a liquid chromatography system for automated purification
Examples and practical protocols are included throughout the handbook to provide a ready-to-use solution or at least a good starting point for further optimization of a specific antibody purification.
The Antibody Purification Handbook is an excellent resource for students and researchers looking to get started in small-scale antibody purification or improve on their existing antibody purification strategy.