Labeling reagents for Western blotting detection
GE Healthcare offers scientists a great selection of chemiluminescent, chemifluorescent and fluorescent substrates, each with distinct advantages, providing powerful solutions to questions posed by today’s research.
 Note: Values of sensitivity reported above come from an experimental model system.
Also available for Western blotting labeling and detection:
ECL GST Western blotting detection kit
ECL Protein biotinylation module and system
ECL phosphorylation module
ECL Glycoprotein detection module
ECF Western blotting reagent
Technical Specifications
TECHNICAL SPECIFICATIONS - ECL Western Blotting Systems |
 | ECL Plex™ | ECL Advance™ | ECL Plus™ | ECL™ |
Sensitivity (pg) | ~ 1 | ~ 1 | ~ 5 | ~ 10 |
Primary antibody usage | 1 : 750 | 1 : 100 000 | 1 : 10 000 | 1 : 1 500 |
Secondary antibody dilution | 1 : 2500 | 1 : 400 000 | 1 : 100 000 | 1 : 10 000 |
Emission duration | > 3 months, protected from light | 4-6 (h) | 24-48 (h) | 1-2 (h) |
Reprobing possible | Not necessary | Yes | Yes | Yes |
Recommended membrane for blotting | Hybond™-LFP
Hybond ECL | Hybond-P
Hybond ECL | Hybond-P | Hybond ECL |
Recommended detection method | Fluorescence scanner | Hyperfilm™ ECL
CCD imaging | Fluorescence Scanner
Hyperfilm ECL
CCD imaging | Hyperfilm ECL, CCD |
|
Kit Components - ECL Western Blotting Detection Reagents |
Product | Detection Reagents | HRP Conjugates | Blocking Agent | Pack Size
(Membrane coverage) |
ECL Advance Western Blotting Detection Kit | X | | X | 1000 cm2 |
ECL Western Blotting Detection Reagents | X | | | 1000 cm2
2000 cm2
4000 cm2
6000 cm2 |
ECL Western Blotting System | X | X | X | 1000 cm2 |
ECL Plus Western Blotting Detection Reagents | X
X | | | 1000 cm2
3000 cm2 |
ECL Plus Western Blotting Reagent Pack | | X | X | Sufficient for 10 mini-blots |
|
Consult the technical support section for contact information
Select another labeling product
See our product selection for each Western blotting workflow step | 
