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Before packing or running the column please make sure that > the tubing, valves, gaskets etc. have the same inner diameter as the column in and outlets and tubing is not longer than needed. > the bed supports have a porosity appropriate for your chromatography medium > the column has been correctly assembled, verified by passing a leakage test > the chromatography medium and equipment are equilibrated in the packing buffer and to ambient temperature of packing environment > that the pump is free from pulsations > no pressure peaks appear > that you have enough slurry volume, taken in account the compression factor of the medium > packing is performed at optimal flow velocity > column meets pressure requirement for selected medium and packing procedure After completing the packing procedure please evaluate the efficiency of the column in terms of the height equivalent to a theoretical plate (HETP), reduced plate number (h) and the peak asymmetry factor (As). Between batches it is recommended to perform cleaning-in-place (CIP) to remove precipitated material, strongly bound substances and other contaminants from the column bed without dismantling the column. Regular CIP is needed to ensure proper product quality and expected life time of medium and equipment. Many issues that face chromatographers are common to all types of columns, while other issues are quite specific. Select the appropriate equipment.
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